Lightsheet Microscopy (LS) also known as Selective Plane Illumination Microscopy (SPIM) allows to perform 3D fluorescence microscopy with a resolution in the micrometer range. In contrast to confocal laser scanning microscopy, instead of scanning the sample point by point a whole plane is illuminated by a sheet of laser light and imaged through a 10x or 20x objective with a CCD camera. This approach allows fast measurements while reducing the local light dose making it advantageous for live cell experiments. There are certain requirements to the sample which should be (i) as transparent (means: refractive index as close to water) as possible, (ii) labelled with fluorescent dyes that can be excited with 405, 488, 561 or 638 nm wavelength and (iii) must not be bigger than ~ 2mm. Samples are measured immersed in water, PBS or cell culture medium.
If you think that the LS microscope might be a useful device for your research, we are happy to support you in this sense by either making test measurements, starting new scientiffic collaborations or training staff in working with the microscope. We can also assist you in questions on sample preparation (the easiest is agarose embedding). The system is not yet equiped for imaging cleared samples but can be upgraded if needed.
FB Chemie und Physik der Materialien
Jakob Haringer Strasse 2a
5020 Salzburg
Raum: UG35
6205 and 6206
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